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Image Search Results
Journal: Journal of Cellular and Molecular Medicine
Article Title: Byakangelicin protects against carbon tetrachloride–induced liver injury and fibrosis in mice
doi: 10.1111/jcmm.15493
Figure Lengend Snippet: Byakangelicin inhibits 4‐HNE–induced hepatocyte apoptosis by inhibiting ASK1/JNK pathway. A, Detection cytotoxicity of byakangelicin in liver stellate cell line LX2 using MTT. B and C, Different time and concentration gradients to detect 4‐HNE–induced hepatocyte HepG2 apoptosis protein full‐length PARP, PARP, cleaved caspase‐3, caspase‐3 and internal reference protein β‐tubulin expression with densitometry. D and E, Protein full‐length PARP, PARP, cleaved caspase‐3, caspase‐3, JNK, P‐JNK, ASK‐1 and P‐ASK‐1 were also detected and quantified using Western blot analyses. F, Immunofluorescence by using antibody against caspase‐3. G, Apoptosis detection by using flow cytometry. For the statistics of each panel in this figure, # P < .05, ## P < .01, ### P < .001; * P < .05, ** P < .01, *** P < .001, n = 3
Article Snippet: We selected HepG2 as
Techniques: Concentration Assay, Expressing, Western Blot, Immunofluorescence, Flow Cytometry
Journal: Journal of Cellular and Molecular Medicine
Article Title: Byakangelicin protects against carbon tetrachloride–induced liver injury and fibrosis in mice
doi: 10.1111/jcmm.15493
Figure Lengend Snippet: Mechanism diagram of byakangelicin attenuating the progression of liver fibrosis. byakangelicin inhibits the proliferation and activation of hepatic stellate cell by inhibiting the TGF/Smad3 signalling pathway and PDGF‐ERK, AKT and Stat3 signalling. In addition, byakangelicin could inhibit 4‐HNE–induced apoptosis and reduce death signalling through the ASK/JNK pathway in hepatocytes
Article Snippet: We selected HepG2 as
Techniques: Activation Assay
Journal: Experimental Biology and Medicine
Article Title: Mesenchymal stem cells alleviate hypoxia-induced oxidative stress and enhance the pro-survival pathways in porcine islets
doi: 10.1177/1535370219844472
Figure Lengend Snippet: IL-6 plays a role in MSCs' protection. (a) ELISA quantification of IL-6 and HGF in MSC-CM. (b) The recombinant human IL-6 (rIL-6) decreased the hypoxia-induced death of NICCs. rIL-6 suppressed total ROS accumulation (c) and augmented protein phosphorylation (d) under hypoxic conditions. nor: normoxia; hypo: hypoxia; CM: conditioned medium. Values are means ± SD. **P < 0.01, ***P < 0.001. (A color version of this figure is available in the online journal.)
Article Snippet: Human IL6 ELISA Kit (
Techniques: Enzyme-linked Immunosorbent Assay, Recombinant, Phospho-proteomics